rabbit anti gpr54  (Bioss)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prognostic value of metastin expression in human pancreatic cancer

doi: 10.1186/1756-9966-28-9

Figure Lengend Snippet: Immunohistochemical staining of non-cancerous pancreatic tissues and pancreatic cancer tissues . (A, B); Immunohistochemical staining of human placental tissues as a positive control. Tissues were stained with anti-metastin (A) and anti-GPR54 antibody (B). (Original magnification, × 200). (C, D); Non-cancerous and cancerous tissues were stained with anti-metastin and anti-GPR54 antibody. (Original magnification, × 400). Weak positivity of non-cancerous ductal cells for metastin (C) and GPR54 (D). (E, F); Pancreatic cancer tissues were stained with anti-metastin and anti-GPR54 antibody. Heterogeneous strong positive immunostaining of carcinoma cells for metastin (E) and GPR54 (F) are shown.

Article Snippet: Nonspecific binding was minimized by incubation with Dako protein block (Dako) for 30 min. Rabbit anti-human polyclonal antibodies for metastin (1–54)-Amide (catalogue number: H-048-59, Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) and GPR54 (375–398) (catalogue number: H-048-61, Phoenix Pharmaceuticals) were applied overnight at 4°C at a dilution of 1:400.

Techniques: Immunohistochemical staining, Staining, Positive Control, Immunostaining

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prognostic value of metastin expression in human pancreatic cancer

doi: 10.1186/1756-9966-28-9

Figure Lengend Snippet: Expression of metastin and GPR54 in pancreatic cancer tissues . Immunoreactivity for metastin and GPR54 in resected pancreatic cancer tissues (n = 53) shown as the intensity score of each patient. The mean metastin intensity score was 72.1 ± 54.9 and that for GPR54 was 99.9 ± 55.1. The horizontal bar indicates the mean ± SD.

Article Snippet: Nonspecific binding was minimized by incubation with Dako protein block (Dako) for 30 min. Rabbit anti-human polyclonal antibodies for metastin (1–54)-Amide (catalogue number: H-048-59, Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) and GPR54 (375–398) (catalogue number: H-048-61, Phoenix Pharmaceuticals) were applied overnight at 4°C at a dilution of 1:400.

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prognostic value of metastin expression in human pancreatic cancer

doi: 10.1186/1756-9966-28-9

Figure Lengend Snippet: Correlation between metastin and GPR54 expression in pancreatic cancer tissues . Scatter plot showing the correlation between immunoreactivity for metastin and GPR54. A strong correlation was found (r = 0.62, p < 0.001).

Article Snippet: Nonspecific binding was minimized by incubation with Dako protein block (Dako) for 30 min. Rabbit anti-human polyclonal antibodies for metastin (1–54)-Amide (catalogue number: H-048-59, Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) and GPR54 (375–398) (catalogue number: H-048-61, Phoenix Pharmaceuticals) were applied overnight at 4°C at a dilution of 1:400.

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prognostic value of metastin expression in human pancreatic cancer

doi: 10.1186/1756-9966-28-9

Figure Lengend Snippet: Comparison of the patients with pancreatic cancer who had positive immunostaining for GPR54 and those negative.

Article Snippet: Nonspecific binding was minimized by incubation with Dako protein block (Dako) for 30 min. Rabbit anti-human polyclonal antibodies for metastin (1–54)-Amide (catalogue number: H-048-59, Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) and GPR54 (375–398) (catalogue number: H-048-61, Phoenix Pharmaceuticals) were applied overnight at 4°C at a dilution of 1:400.

Techniques: Immunostaining

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prognostic value of metastin expression in human pancreatic cancer

doi: 10.1186/1756-9966-28-9

Figure Lengend Snippet: The rate and site of recurrence after resection of pancreatic cancer in relation to GPR54 expression.

Article Snippet: Nonspecific binding was minimized by incubation with Dako protein block (Dako) for 30 min. Rabbit anti-human polyclonal antibodies for metastin (1–54)-Amide (catalogue number: H-048-59, Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) and GPR54 (375–398) (catalogue number: H-048-61, Phoenix Pharmaceuticals) were applied overnight at 4°C at a dilution of 1:400.

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prognostic value of metastin expression in human pancreatic cancer

doi: 10.1186/1756-9966-28-9

Figure Lengend Snippet: Impact of GPR54 expression on survival time of pancreatic cancer patients . Overall survival of patients whose tumors were positive (n = 30) or negative (n = 23) for GPR54 immunostaining. The survival of patients with tumors positive for GPR54 was significantly longer than that of those with negative tumors (p = 0.02).

Article Snippet: Nonspecific binding was minimized by incubation with Dako protein block (Dako) for 30 min. Rabbit anti-human polyclonal antibodies for metastin (1–54)-Amide (catalogue number: H-048-59, Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) and GPR54 (375–398) (catalogue number: H-048-61, Phoenix Pharmaceuticals) were applied overnight at 4°C at a dilution of 1:400.

Techniques: Expressing, Immunostaining

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prognostic value of metastin expression in human pancreatic cancer

doi: 10.1186/1756-9966-28-9

Figure Lengend Snippet: Univariate and Multivariate analyses of factors associated with survival after resection in patients with pancreatic cancer.

Article Snippet: Nonspecific binding was minimized by incubation with Dako protein block (Dako) for 30 min. Rabbit anti-human polyclonal antibodies for metastin (1–54)-Amide (catalogue number: H-048-59, Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA) and GPR54 (375–398) (catalogue number: H-048-61, Phoenix Pharmaceuticals) were applied overnight at 4°C at a dilution of 1:400.

Techniques: Expressing

Journal: iScience

Article Title: The E3 ubiquitin ligase RNF216/TRIAD3 is a key coordinator of the hypothalamic-pituitary-gonadal axis

doi: 10.1016/j.isci.2022.104386

Figure Lengend Snippet:

Article Snippet: Membranes were then probed with the following primary antibodies prepared in a 1:1 ratio of TBST (1X TBS, 0.1% Tween-20) and blocking buffer solution with 1:1,000 20% NaN 3 : rabbit polyclonal anti-RNF216 (Bethyl Laboratories, 1:1,000), rabbit anti-KISS1R/GPR54 (Lifespan Biosciences, 1:600), rabbit anti-GNRHR (Lifespan Biosciences, 1:600), rabbit anti-Arc (Synaptic Systems, 1:1,000), rabbit anti-GFP (Fisher, 1:1000), or mouse anti-β-Actin (Genetex, 1:3,000) and were incubated overnight at 4°C.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Imaging, Recombinant, Modification, Blocking Assay, Electron Microscopy, Plasmid Preparation, Gel Extraction, Transfection, Polymerase Chain Reaction, Staining, Ligation, Mutagenesis, CRISPR, Clone Assay, Sequencing, SYBR Green Assay, shRNA, Software